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foreign welcome back to histological techniques at qet this week back in the lab and staining for iron pigment using pearlstone so to begin weamp;#39;ve already taken some slides down to water by the paraphernizing in xylene and taking through the grated alcohols and once weamp;#39;ve done that we want to give those slides a good rinse in deionized water especially for a stain of this type where if there are traces of iron in the water that could lead to contamination issues so to begin after rinsing the slides well we need to prepare our solution for staining and itamp;#39;s in equal parts of two percent hydrochloric acid now on this occasion I have around eight slides there to be stained so if we just measure out approximately four mils of the hydrochloric acid we can then combine that with an equal volume of the potassium ferrocyanide letamp;#39;s get rid of that right so the next reagent is the potassium ferrocyanide and itamp;#39;s important to always check that you h