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[music] Hey everyone, welcome to another Restek tip. Today we are going to talk about troubleshooting LC retention time shift. Retention times must be reproducible from run to run. When problems occur, late, early or variable retention times may be observed. Lets say we are doing some liquid chromatography and we start seeing retention time shifts over subsequent runs. One possible reason for this could be a leak in the system. If there is no leak, then check the flow rate. Sometimes inadequate degassing, faulty check valves and pump seal failure may also cause flow instability. Temperature fluctuations can also cause shifts in retention time, therefore it is important to use a thermostatted column compartment to maintain the column temperature throughout the day. You should always strive to match the aqueous to organic ratio as well as the buffer strength of the sample diluent to the start of the gradient. If we do not, it can affect analyte retention time and peak shape. Another rea