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hello everyone so today Im going to be showing you how you can use our spot map software in fact any of our software to adjust the contrast of your images to allow you to see faint or weaker spots or bands that you might be interested in measuring hello everyone so today I was asked a question by a customer how do I visualize my very faint spots within my experiment without losing the accuracy with my very strong spots and its a very good question it comes down to visualization of the image so they asked should I do that in my capture step should I have a longer exposure so that I can see those faint spots and I said no because the longer exposure you have to try and get faint spots youll likely extend the dynamic range of the instrument that youre using when it comes to your fate to your strong spot sorry and you want to be able to consider both so what you want to do is start with an image within the dynamic range of your Imaging equipment and then allow the software to do the vi