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this protocol can be used to measure patterns of breathing in mice importantly it can minimize the impact of active behaviors and genetically modified strains that can confound results the main advantages of this technique are that it is non-invasive and that it does not require the use of anesthetic or stress inducing restraints begin by making sure that all of the hoses and tubes are connected to the barometric plathismograph chamber and by connecting a gas inflow tube and a vacuum outflow tube directly to the barometric plethysmography chamber to calibrate the low flow into the chamber set the flow into and out of the chamber to zero and remove the flow tube into the chamber turn off the vacuum and enter a zero into the low unit cell to record the zero flow in the 7700 amplifier setup of the barometric plathismography software then reattach the inflow tube and turn on vacuum flow to calibrate the high flow and to allow 20.93 oxygen balanced nitrogen gas to flow through the b
