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hello in this brief tutorial Iamp;#39;m going to be using image J software to analyze some control micro taken of cardiac muscle so this is image j and i have it loaded on my mac computer and this is an image taken staining using the wheat germ agglutinin stain using alexa fluor 488 that allows me to visualize the cell membranes of cardiac myocytes this is also be counter stained with Dappy so i can see the nuclei if i drag the image to the software it loads up the image in image j and allows me to freely view the image image j allows a lot of image manipulation but for this purpose Iamp;#39;m just going to use it to do some simple counting and some simple area measurement allowing it also to calibrate the software to the correct area so the image has already got a scale bar between naught and 50 micrometers and so I want to first calibrate image J to accept this distance as 50 micrometers the way we do this is in the analyse window we can set scale and in the set scale we can type a
