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foreign here is an image of cells stained in green and nucleus in blue we will use image J software to segment the cells from one another count the number of cells and measure their areas open the image of Interest using image J software the first step is to change the image type into an 8-bit image change the brightness and contrast of the image if required before changing the image type since we require two of the same images therefore create a duplicate copy now we have two images of the same select the first image and click on process and find Maxima in the fine Maxima window select the preview Point selection a number of points are selected in the image in the output type select segmented particles to perform a correct selection of the cells in the image change the value in the prominence select the prominence such that all the individual cells are marked once the parameter is set click OK the output is a mass image save this image by clicking on file save us and select any format
