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advancements in genomics and proteomics over the past two decades have propelled assay development to ever increasing levels of sensitivity and sophistication however one challenge has remained the continuous measurement of morphological chemical and physiological change in live cell populations over time this window of opportunity in the research field is attractive because it is much less expensive than live animal and organ models yet can deliver docHubly better information than studies involving dead or fixed cells though techniques exist to isolate study and measure individual cells they rely on end point measurements within a typical experiment cells are introduced onto a growth medium and are then subjected to various treatments stimuli agonists or promoters treatment or stimuli can range from the binding of a ligand to a receptor the infection of a cell with a virus the transfection of a cell with foreign dna or rna a change in the environmental conditions exposure to a th