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this protocol is docHub because it enables users to develop customizable multi-taper spectrograms without prior signal processing knowledge the main technical advantage of the program is the programamp;#39;s user-friendly design and the ability to create multi-taper spectrograms using computers without matlab licensing seven to ten days after the electrode implantation surgery configure the data acquisition system to record all of the signals in millivolts and obtain eeg recordings for the desired experimental duration amplify and digitize the unfiltered eeg signals using the appropriate data acquisition instrumentation and software then have two different individuals independently score each 10 second bin of the digital eeg blue trace and emg black trace recordings as wakefulness rem sleep or non-rem sleep in an appropriate sleep scoring software program download the compiled multi-taper spectrogram program for spectrogram computation obtain raw unprocessed eeg data in e
