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In the last lecture, we discussed sandwich ELISA, where a target antigen is positioned between a capture antibody and a detection antibody. An application of this method is in home pregnancy test kits. These kits have a test strip with a test zone (T) and a control zone (C). When a urine sample is applied, two results can occur: if both T and C lines are colored, the test is positive; if only the C line appears, the test is negative. Today's lecture will explore what urine components these pregnancy strips detect, the biochemical substances involved, and the operation of these test strips. We will begin by identifying the specific hormone that pregnancy test kits detect and confirm.