When you need to apply a minor tweak to the document, it must not require much time to Establish columns license. This type of simple action does not have to demand extra training or running through manuals to learn it. Using the appropriate document modifying tool, you will not spend more time than is needed for such a swift change. Use DocHub to streamline your modifying process whether you are a skilled user or if it’s the first time making use of an online editor service. This instrument will require minutes or so to figure out how to Establish columns license. The only thing required to get more productive with editing is actually a DocHub profile.
A plain document editor like DocHub can help you optimize the amount of time you need to spend on document modifying regardless of your previous knowledge of this kind of tools. Create an account now and increase your productivity immediately with DocHub!
[ Music ] Today we are talking about column chromatography. Everything I do, should be done in a hood because you are using volatile organic solvents. So, for video purposes we are doing this on the benchtop but remember always work in a hood. You are already familiar with a TLC which is a separation technique that uses silica gel chromatography. TLC is an analytical technique meaning all you get out of this technique is information. We are using way to little compound for it to be practically useful. If you want to have a sizeable amount of compound you have to scale it up. So, to scale it up, you dont use just a very thin layer of silica on a plastic backing. You use a lot of silica and you pack a column of it. Column chromatography is a very touchy technique. There are three phases in column chromatography. First, you pack the column. That is, you prepare it for you experiment. Then, you load it. Which is the equivalent of spotting the TLC plate that is you put your compound