With DocHub, you can quickly embed stain in WRF from anywhere. Enjoy capabilities like drag and drop fields, editable textual content, images, and comments. You can collect electronic signatures securely, include an additional layer of defense with an Encrypted Folder, and collaborate with teammates in real-time through your DocHub account. Make adjustments to your WRF files online without downloading, scanning, printing or sending anything.
You can find your edited record in the Documents folder of your account. Create, share, print, or convert your document into a reusable template. Considering the variety of powerful features, it’s simple to enjoy seamless document editing and management with DocHub.
load the paraffin-embedded tissue onto a microtome and cut into five micrometer sections transfer the tissue to a bath of distilled water at 40 degrees Celsius and mount on a glass slide suitable for immunostaining dry slides overnight at 56 degrees Celsius follow the slide treatment steps as listed here xylene solution 3 times 5 minutes each 100 percent ethanol 3 times 5 minutes each 95 percent ethanol for 3 minutes 70 percent ethanol for 3 minutes ro/di reverse osmosis and deionization and water 3 times 1 minute each for chroma genic staining xamp;#39; block endogenous peroxidase activity by incubating slides in 3% hydrogen peroxide solution in methanol at room temperature for 15 minutes perform antigen retrieval using a microwave or a pressure cooker antigen retrieval with a microwave prepare a 100 mil solution of 1x citrate buffer by mixing 10 mils of bio legends 10x citrate buffer catalog number 4 2 0 9 0 1 with 90 mils of distilled water microwave
