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The overall goal of this video is to demonstrate an immunohistochemistry protocol for paraffin-embedded tissue sections. This is accomplished by first slicing paraffin infiltrated tissue on a microtome and then mounting the sections onto charged slides. The sections are then deparaffinized and rehydrated before performing antigen unmasking to remove the cross-links formed by fixation. Next, a specific antibody is applied to the sections to bind to its target epitope prior to chromogenic staining. After dehydrating the sections, the final step is to mount the sections for microscopic analysis. Ultimately, antibodies are used to detect and analyze protein expression while maintaining the composition, cellular characteristics, and structure of native tissue. Generally, individuals new to this method will struggle because there are so many steps in the procedure and variations in each step can have an impact on staining. (CST) antibodies that are validated for immunohistochemistry are deve