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hello and welcome to this demonstration of gordon and sweets reticular stain so the first step in this staining protocol involves treatment of the slides with an acidified solution of potassium permanganate the popular theory is that this will actually oxidize some of the glycoproteins associated with the reticular fibres now having treated the tissue with the potassium permanganate when you rinse it off you will notice that there is a color change and in order to remove that color from the section you can just pick up a little bit there even on my blue glove we then subsequently treat the slide for a further one to two minutes in oxalic acid theoretically this might cause some further oxidation but thereamp;#39;s not much evidence for that okay so just put it on give a bit of a shake until itamp;#39;s pretty much lost its color then we rinse that off in tap water followed by deionized water so this is the first critical step now where itamp;#39;s essential to start removing