Embed stain in DWD

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Aug 6th, 2022
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How to embed stain in DWD

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The staining procedure for HE follows a basic protocol: Dewaxing. Dehydration. Hematoxylin. Differentiation. Bluing. Eosin. Dehydration. Clearing.
Method - Hematoxylin is dissolved in distilled water using gentle heat. Then alum is added and dissolved. Then sodium iodate, citric acid and chloral hydrate are added respectively. Eosin is used as the counterstain that stains the cytoplasm rose coloured.
Stain in Harris Hematoxylin Solution for 2.0 to 2.5 minutes. 4. Rinse slide in running tap water.
In the following sections, the basic steps in performing an HE stain are outlined. Remove the Wax. Hydrate the Section. Apply the Hematoxylin Nuclear Stain. Complete the Nuclear Stain by Blueing Remove Excess Background Stain (Differentiate) Apply the Eosin Counterstain.
Manevals solution (a mixture of acetic acid and acid fuchsin) is added to the slide. The acetic acid lowers the pH in the sample and causes the Congo red to change from a red color to blue. The acid fuchsin (a basic dye) interacts with the bacterial cell, staining the cell bright red.
A mordant is a chemical that serves as a link between the dye and the substrate. The result is an insoluble compound that helps adhere the dye to the cells. The most useful mordants for hematoxylin are salts of aluminum, iron, tungsten, and occasionally lead.

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