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so here we have two slides that have been taken down to water for the purpose of staining with the PAS method so the P of course stands for periodic acid thatamp;#39;s an oxidizing agent and weamp;#39;ll be applying that to a section of liver and to a section of kidney so quite different in terms of what we expect to see just give these a bit of a rinse with deionized water itamp;#39;s less critical at this point but since there are chemical reactions taking place it doesnamp;#39;t hurt to have something fairly clean on the on the slide so as flick that off and then take our periodic acid now the length of time that we need it does differ depending upon what weamp;#39;re staining for something like liver where we may have glycogen about 10 minutes is sufficient and for kidney weamp;#39;re mainly looking at basement membranes so weamp;#39;d need to give that a bit longer up to about 20 minutes but just for the purpose I think of this demonstration for both this one and th
