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this video is an overview of the steps taken in preparing tissue samples for light microscopy this is a view down a microscope of a thin section of kidney the kidney has been fixed processed sectioned and stained to show its cellular structure if you try to cut a piece of fr tissue and look at it with the microscope all that can be seen is a structureless blob there are several obvious problems it is not permanent so the tissue decays it is difficult to cut sections thin enough to allow light to pass through the Section and it lacks color these difficulties are overcome by fixation or preservation provision of a support medium for the tissue and staining to preserve fresh tissue it is placed in fixative for a length of time suitable for the size of the piece and the type of fixative used for example a specimen of this size would be fixed in formon for approximately 24 hours and then stored in 70% alcohol the original sample is trimmed transferred into a label plastic cassette and then
