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the overall goal of this procedure is to use immunohistochemical staining techniques to evaluate the expression of b7h1 pdl1 on paraffin sections of human pancreatic adenocarcinoma tissue this is accomplished by first removing the paraffin from and rehydrating the sliced tissue sections the second step of the procedure is antigen retrieval which involves the use of a pressure cooker to stain the tissue an antibody staining procedure is followed by signal amplification and dav color development steps the final steps are to dehydrate and mount the tissue once complete microscopy and image analysis can be used to visualize membranous and cytoplasmic b7h1 this method can help answer key questions in the study of a pancreatic endocarcinoma by giving us a better understanding of how b7h1 btl1 contributes to the suppression of anti-tumor immunity in the tumoramp;#39;s environment the main advantage of this technique over existing methods such as staining on cryo sections is that it h
