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the social cryo vial offers flexibility and use compared to other cryo storage containers to fill the vial use a standard luer lock syringe draw a small volume of air into the barrel of the syringe and aspirate the desired volume of fluid to fill to fill the vial connect the lower lock to the fitting at the top of the fill to ensure the connection is if resistance is felt during film ensure that the connection is completely closed by twisting an additional quarter turn slowly depress the plunger to fill the vial continue depressing through the extra air to evacuate the filter the clear access cell seal file allows filling using a syringe or a pipette tip completely depress the pipette into the open fill tube and slowly dispense the fluid because the tube will be open gravity will evacuate the victim to prepare vials for cryogenic storage seal the tubes using cell seal RF seal place one tube between the jaws of the sealer and depress both the jaws closed for an additional three seconds