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foreign acquired resistance mechanisms will contribute to the development of more effective and safe therapeutic strategies in patients with uncancer agent resistant and resectable cancer unfortunately cases in which drug resistance failed to develop are generally not reported this stepwise does the exploration method is considered the most reliable technique for obtaining acquired resistance cells to determine the appropriate of fat nib resistance concentration culture pc9 cells in growth medium in a 10 centimeter cell culture treated dish at 37 degrees Celsius and 5 carbon dioxide we suspend the pc9 cells at a 4 times 10 to the 4 cells per milliliter of growth medium concentration and Seed the cells at 50 microliters of cell suspension per well in a 96 well microplate after an overnight incubation in the cell culture incubator treat the cells with 50 microliters of a fat nip solution per well at six replicates of the indicated concentrations and after a 96 hour incubation in
