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here second is about how to use the reference frame so two reference frame is one of the functions in Maris to let you do to menu add a new coordinate system so you can measure the statistic relative to position pets on this new coordinate system so here let me show you okay since we are in this image I just quickly go through everything okay so I try to create a new surface for red structure and I create a spot for my green structure green sails okay lets about it alright so follow the surface to spark colocalization we can use here and add to the spot and the eighth shot is this into surface then we can turn off turn on then say whatever numbers we want five so there is probably no one within five microns only one inside so using the same distance short native shortest distance calculation you will be able to filter out this spot within a specific distance to the service object you want like this okay then folder reference frame one of the useful area for reference frame is to to s