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[Music] hi and welcome back to freesciencelessons.co.uk by the end of this video you should be able to describe how to use quadrats and transects to determine the abundance and distribution of species in an ecosystem in a recent video we saw that living organisms are affected by a number of abiotic factors and remember that abiotic factors are not living abiotic factors include the light intensity the temperature and the levels of moisture the levels of abiotic factors can vary from place to place and this affects the number of different organisms that we find in any location so in this video were looking at how scientists determine the number of organisms in an area to do this scientists use a technique called sampling and there are two ways to do this these are called random sampling unsampling along a transect were going to start by looking at random sampling random sampling is used to compare the numbers of organisms in different areas to do this we use a quadrat and im showing