Darken stain in PAP smoothly

Aug 6th, 2022
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How to darken stain in PAP with top efficiency

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How to Darken stain in PAP

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hi everyone for todays topic we will be discussing about the papa nicolao staining technique but before that i want you to know that papa nicolau stain or the pop staining method is under the big umbrella of diagnostic cytology under diagnostic cytology it also belongs under exfoliative cytology so lets differentiate first what diagnostic cytology and exfoliative cytology means diagnostic cytology class simply means microscopic examination of cells from different body sites for diagnostic purposes diagnostic cytology includes exfoliative abrasive and aspirational but for today lets focus on exfoliative cytology deals with the microscopic study of cells that have been disqualmated from epithelial surfaces exfoliated cells may be found in smears that have spontaneously been shed or physically removed from epithelial and mucous membranes such as the vagina bucal mucosa and from body fluids such as the sputum urine plural peritoneal or acetic fluid the gastric juice and cerebrospinal f

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[2] The Pap stain has been most frequently used for screening of cervical cancer.
The modified PAP stain showed a positive staining of KP in 30 (78.9%) cases, whereas HE stain showed positive staining in 20(52.6%) cases (Table-1, Graph-1).
Papanicolaous stain (PAP stain) is the most important stain in cytology and is used in all cervical smear and non-gynaecologic exfoliative smears.
The conventional Pap stain has 24 solutions, of which three are acid dyes, six water, one is chemically defined bluing agent, eight 95% ethanol, three absolute alcohol and three xylene Gill, 2006.
The PAP stain is a polychromatic counterstaining method consisting of stains such as Orange G 6 (OG6) and modified eosin azure (EA). The strength of the Pap stain is such that it results in: Well-stained nuclear chromatin. Differential polychromatic counterstaining of cytoplasm.
Because the Papanicolaou stain was developed to document keratinization (the orange G dye component binds avidly to keratin), it is supe- rior to Romanowsky-type stains for this purpose. Identification of cytoplasmic keratinization may be essential for diagnosing squamous cell carcinomas.
A method for staining a Pap test slide specimen for conventional Pap test and, particularly a ThinPrep Pap test specimen, in which a number of multiple hydrating, staining and dehydrating step are performed in an equal number of 10 to 15 dippings at about equal dip times of about 1 second.
Pap stain is a universal stain used for gynecologic and non-gynecologic cytology smear. It is mainly used for oral and cervical cancer screening in asymptomatic population and in the follow up of patients with cancer. Pap test has decreased incidence of cervical cancer by 70% in developed countries.
The modified PAP stain showed a positive staining of KP in 30 (78.9%) cases, whereas HE stain showed positive staining in 20(52.6%) cases (Table-1, Graph-1).
Papanicolaou stain (also Papanicolaous stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.

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