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hi everyone for todays topic we will be discussing about the papa nicolao staining technique but before that i want you to know that papa nicolau stain or the pop staining method is under the big umbrella of diagnostic cytology under diagnostic cytology it also belongs under exfoliative cytology so lets differentiate first what diagnostic cytology and exfoliative cytology means diagnostic cytology class simply means microscopic examination of cells from different body sites for diagnostic purposes diagnostic cytology includes exfoliative abrasive and aspirational but for today lets focus on exfoliative cytology deals with the microscopic study of cells that have been disqualmated from epithelial surfaces exfoliated cells may be found in smears that have spontaneously been shed or physically removed from epithelial and mucous membranes such as the vagina bucal mucosa and from body fluids such as the sputum urine plural peritoneal or acetic fluid the gastric juice and cerebrospinal f