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this video focuses on dark field illumination for this video I will be using 80 490 microscope dark field illumination is a lighting method used to increase contrast in low contrast samples itamp;#39;s particularly useful for highlighting specific sample material while other methods such as phase contrast are better for viewing interior details at the heart of dark field illumination is a dark field condenser it works similarly to a bright yo condenser except it includes an opaque disc or stop which blocks direct light from entering the objective lens instead only oblique lighting which is refracted by the sample will enter this results in certain objects being illuminated while the background remains dark when choosing a dark field condenser be sure the condensers numerical aperture is larger than a numerical aperture of the objective lens you plan to use if the objective lenses aperture is larger than the condensers direct light will be transmitted and a dark field will not be achie
