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welcome to ask TaqMan this is where we answer your questions about real-time PCR hereamp;#39;s a good question I get a lot whatamp;#39;s this rocks I keep hearing about and whatamp;#39;s its function in real-time PCR well much like fam Vic in cyber green rocks is a fluorescent molecule that the real-time instrument can detect when itamp;#39;s present in the reactions but unlike those other dyes rocks is a passive dye meaning itamp;#39;s fluorescent level doesnamp;#39;t change as a direct result of the amplification instead rocks just sits there now you may be asking whatamp;#39;s the point well within any real-time PCR system there are several potential sources of well - well variability in the excitation of the reporter molecule and therefore in the detection of signal from the reporter these include uneven illumination slight variations in the optics from well to well and even differences in the amount of condensation rocked normalizes for these and other sources of variabilit
