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this tutorial will take us through the steps of doing the area of staying analysis here we want to analyze the brighter parts of this tissue section coats a threshold image adjust threshold use the type of thresholds analyzed using Auto threshold function the previous tutorial we got to analyze set measurements to define the measurements we want to read out in this case area mean gray value and area fraction and we make sure that weamp;#39;re quantifying using the threshold click OK we can then go back to back to analyze measure or control M and that gives us readout for the area in pixels in this case the mean intensity value and the percentage area of the whole image