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thank you the overall goal of this procedure is to determine the lipid droplet index of cells cultured under different nutritional conditions this methods can help answer key questions in the lipid droplet in lipid field such has the dynamic of accumulation under different nutritional conditions the main advantage of this technique is that multiple samples can be read simultaneously because it can be implemented in a microplate we first talk of this method when we observe reflectance of stilago midi cells cultured under nitrogen starvation and then realized Elijahamp;#39;s meeting that these structures might be lipid droplets so we contacted Monica Montero from University Federal in Brazil Who implemented the liquid fluorescence recovery aside of lipid droplets in Sacramento television to start a collaboration to adapt the procedure for stilagamites the first step in this protocol is the preparation of the required buffers and solutions to prepare a 10 millimolar bodp
