Darken identification in PAP

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Aug 6th, 2022
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PAP may not always be the simplest with which to work. Even though many editing tools are out there, not all offer a easy solution. We designed DocHub to make editing effortless, no matter the file format. With DocHub, you can quickly and easily darken identification in PAP. Additionally, DocHub gives a variety of additional tools such as form generation, automation and management, industry-compliant eSignature tools, and integrations.

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To darken identification in PAP, follow these steps:

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  2. When forwarded to your Dashboard, click the Add New button and select how you want to add your file.
  3. Use our advanced tools that can help you improve your document's text and layout.
  4. Choose the ability to darken identification in PAP from the toolbar and use it on form.
  5. Check your text once again to make sure it has no errors or typos.
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How to darken identification in PAP

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here is a hyperchromatic crowded group that we can easily recognize on low power and um when we look carefully we can see that the nuclei are very jumbled up and theyamp;#39;re very disorganized taking a closer look uh the nuclei are overlapping each other there is actually a fair variation in nuclear size and shape some nuclear are much larger than others they appear to be very hyperchromatic there is no definite gland formation seen even in this uh cell group we can see that the nuclear are enlarged and hyperchromatic with irregular nuclear membranes so these are features that suggest scamers differentiation here is another hyperchromatic crowded group and we can see the nuclei are quite elongated if you look at the edges of the group um here and especially here there is a hint that the nuclear are sort of flattening out so that they are parallel to the edges of the group rather than perpendicular uh this parallel Arrangement is another feature that suggests squamous differentiation

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Papanicolaou stain (also Papanicolaous stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.
The principle of pap stain Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid). Orange G is an acid dye and stains keratin with a bright intense orange.
Composition of the Reagents of Papanicolaou Staining (Pap stain) Hematoxylin = 2.5g. Ethanol = 25ml. Potassium alum = 50g. Distilled water (50C) = 500ml. Mercuric oxide = 1-3g. Glacial acetic acid = 20ml.
The Papanicolaou stain is undoubtedly the most commonly used in cytology. It has three cytoplasmic dyes, orange G, eosin Y, and light blue. It can stain cells blue or blue-green (basophilia, cyanophilia), pink (acidophilia, eosinophilia), orange (orangeophilia), or indeterminate (gray-blue).
Atypical squamous cells of undetermined significance (ASC-US): This is the most common abnormal Pap test finding. It means that some cells dont look completely normal, but its not clear if the changes are caused by HPV infection.
Staining techniques The Papanicolaou stain (PAP) requires immediate fixation in alcohol. Nuclear chromatin patterns are easily discerned and the degree of maturation/keratinisation of squamous cells is readily appreciated. Mucin may not be seen so easily. May-Grnwald Giemsa (MGG) staining is used on air-dried smears.
Papanicolaou achieved this polychromasia by introducing 2 distinct cytoplasmic counter stains into the staining method: orange (O)G6 and either EA36 or EA25. In doing so, the Pap stain protocol then incorporated 5 distinct dyes as EA consisted of light green, Bismarck brown, and eosin.
Common tumor markers for cervical cancer include: cancer antigen 125 (CA 125) carcinoembryonic antigen (CEA) cancer antigen 19-9 (CA 19-9)

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