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the overall goal of the following experiment is to utilize fluorescent dyes to monitor changes in membrane dynamics and intracellular ion concentrations in a bacterial system this is achieved by first incubating bacteria during their growth phase with the fluorophore of interest until it has equilibrated across their membranes next the bacteria are treated with the agent of choice and their fluorescence is monitored the results can show changes in membrane depolarization membrane integrity and intracellular ion concentrations based on the observed changes in fluorescence of the indicator dyes the main advantage of this technique over conventional electrophysiology techniques like patch clamping that is used in eukaryotic systems is that changes in membrane dynamics and intracellular ion concentrations can now be monitored in bacteria using fluorescence generally individuals new to this method will struggle because it takes some effort to optimize the dye concentrations loading
