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welcome back uh so I figured for the last Sky Jam we would do something that involved another group comparison and weamp;#39;ll actually uh interrogate some DDA data uh because as Lucas mentioned we still as Labs generally generate a lot of DDA data and if you want to look at it quantitatively uh thatamp;#39;s possible in Skyline and itamp;#39;s sort of the one thing we havenamp;#39;t covered um so so yeah so the uh this is the the set of methods that weamp;#39;ve said that Skyline can support all chromatography based quantification uh SRM we did PRM and weamp;#39;ve been discussing Dia data independent uh swath uh with Skyline and then finally uh ms1 chromatogram extraction from DDA data which is what weamp;#39;re going to talk about now so our one our one opportunity to talk about that that just a little bit uh in general I I prefer uh when uh to to get as quickly as possible to this you know hypothesis testing mode uh you can do the same types of profiling experiments that Lu