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all right in this tutorial we will be reviewing how one can acquire a fluorescent image using standard techniques on the aura software platform provided by si imaging in our logo amy htx and kino systems now i happen to be on an amy hdx system right now but the protocols to be presented can be applied in the lago or logo x system as well as in the kino systems that we provide so we are on the home page of aura here and to get started go down to the bottom left hand corner and you will see that there are four tabs each designating a different type of imaging that you can do in aura now weamp;#39;re going to do standard fluorescent imaging so thatamp;#39;s going to require us to select the fluorescence tab before doing so iamp;#39;d like to draw your attention to the fact that we are in the luminescence setting mode right now and we have the camera ribbon off to the right with all of the various different camera parameters associated with bioluminescent imaging now when you do