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[Music] since its inception more than three decades ago chromatin immunoprecipitation or chip has become the most widely used method for chromatin mapping it offers powerful insights into the epigenetic mechanisms associated with human diseases including many types of cancer but its low resolution high background and expensive price tag makes chip less than ideal in response researchers have developed new immuno-tethering strategies which are gaining widespread popularity in the chromatin field the next advance in these strategies is cut and tag a novel assay under epiciphers katana platform for ultra-sensitive genomic mapping approaches cut and tag stands for cleavage under targets and tagmentation in cut and tag extracted nuclei are immobilized on a solid support and then treated with a primary and secondary antibody to label a histone post-translational modification or ptm or to label a chromatin-associated protein of interest next the nuclei are treated with a fusion protein made
