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(upbeat music) - [Narrator] Dynamic interactions between proteins and DNA drive many biological processes, including expression of genes that contribute to cell differentiation, development, and progression of diseases. Mapping where and when these protein-DNA binding events occur is essential for mechanistic studies of epigenetics in both normal development and disease. To generate this kind of data, scientists routinely use chromatin immunoprecipitation. This involves a three-day-long protocol including steps for chromatin cross-linking and fragmentation, immunoprecipitation, isolation and elution of complexes, and DNA purification. Now, a newer approach, known as CUTRUN, is available. How does CUTRUN work, and how is it different from ChIP? To prepare the input material for CUTRUN, cells are harvested, bound to magnetic beads to simplify handling and minimize sample loss and permeabilized with digitonin. No crosslinking fixative is needed, so the chromatin is kept in its native