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Hello, my name is Ellen Weinzapfel and welcome to EpiCyphers CUTANA CUTRUN protocol video. This video will be broken up into four sections. First, I will give a brief overview of how CUTRUN works and review important considerations for experimental design and sample preparation. Then I will give a walk-through of the protocol. Next, I will go over protocol variations and wrap up with a few frequently asked questions. CUTRUN stands for Cleavage Under Targets Release Using Nuclease. Concanavalin A or ConA beads coated with lectin proteins are used to bind the glyco-conjugates found on the membranes of cells or nuclei. The membrane is permeabilized, and chromatin is flagged with antibodies for the target of interest, in this case to a histone PTM. Next, Protein AG-Micrococcal Nuclease (also known as pAG-MNase) is added, which binds to antibody-bound chromatin. The addition of calcium chloride activates the enzymes cleavage of the target complex which is then able to diffuse out of