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hi Iamp;#39;m doctor Leo Chen from next salon bioscience today I am presenting a label free direct cell counting method to normalize your seahorse accept data using image cytometry one of the critical factors to ensure accurate seahorse analysis results is uniform self-seeding density what happens if seeding is not uniform currently researchers normalized seahorse results using protein analysis assays such as pca to minimize the effects of non-uniform cell seeding the assay requires preparation of protein standards as well as pca working reagents next the test samples are mixed with the working reagents and then incubated for 30 minutes at 37 degrees finally the mixture is cooled and the protein contents are analyzed using a spectrophotometer protein analysis is a widely used method due to its availability in biological laboratories however it is time-consuming taking usually 45 minutes to complete the assay requires multiple steps of washing licensing and sample transfer this can ris
