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Hello! Tests are not perfect. They can give a false positive result or a false negative one. For example, false negative results can be frequent with available COVID-19 tests if someone has been infected less than 14 days ago You can read more about it in this manuscript where this figure comes from. But why would a test give false results? Tests measure a biomarker, for example antibodies, and if the measurement exceeds a pre-specified threshold - what we call the cut off - the result is declared positive and if it doesnt, negative. Guess what. The value of the biomarker is not always different between healthy and infected individuals. There is a gray zone where values can belong to either healthy or infected ones. Hence, whatever the cut off there will always be some false results, false positive or false negative or both. So how do you pick a cut-off? You may want to be sure that everyone infected tests positive. In this case you place the cut-off here. Or if you want to make su