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the threshold then weamp;#39;ve looked at itamp;#39;s one of the key parameters as well to do with the intensity of steaming was used to check nuclei the maximum background intensity itamp;#39;s really if you beyond the background subtraction then that gives us a local estimate of the background everywhere across the image and if thatamp;#39;s very high itamp;#39;s basically telling Cuba not to apply detection where the background is very high that can be slightly useful if you have some dark tissue falls within the image you can use as much max background intensity value to reduce the likelihood of detecting cells which cannot be adequately measured within these tissue folds to do that you might need to adjust the value because the minute itamp;#39;s at a pretty pretty high value and so it doesnamp;#39;t tend to have much effect with the default
