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hello wanted to come on here and show you how to process Department of Transportation and non-department of Transportation Federal and non-federal urine specimen collection for drug testing so your custody and control form at the top will say Federal or non-federal that is the difference in between Dot and non-dot specimen collection youamp;#39;re going to have your transport bag and also your specimen now when you have them pee in a cup make sure you tell them to provide a sample above the temperature strip okay and the biggest difference in between uh specimens collected for DLT and non-dot split con collection non-dlt can request this but dot is a definite 45 ml collection okay the line here is 15 mL the line here is 30 ml so when youamp;#39;re doing a federal DOT collection you need a split collection both bottles youamp;#39;re going to do 30 in this one 15 in this one there will be labels for bottle a for bottle B but the most important thing is to make sure that youamp;#39;re