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in this video clip I will show you and explain to you step by step how to analyze data taken from a Elisa essay and how to construct a linear standard curve in graph pad prisms and then show you how to calculate the concentration of unknown proteins and how to build a part chart to display the outcome and how to determine the docHub difference between groups here in this Excel sheet I had inserted the design for the plate 96 well plate that has a pin used in Eliza experiment and inserted in the Elisa Plate Reader as shown here there are 96 Wells the first two columns are had placed the standard curve samples in duplicates and these samples were serially diluted up to cell number G as labeled here G and on the same columns also I had placed a plank which is just water and also the control and the disease were color-coded as shown here um the light pinkish is for the disease samples and the light green is for control and the standard samples is given uh light brown color and