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a pregnancy test works by measuring a specific hormone in the body human chorionic gonadotropin or HCG about six days after fertilization the human embryo has multiplied from one to two to four and eventually into a multicellular organism called a blastocyst the blastocyst then implants or attaches to the lining of the uterine wall or endometrium after implantation the body begins to produce the hormone HCG HCG levels rise fast and can be measured either through a blood test or a urine test home pregnancy tests use urine samples to test HCG levels the pregnancy test contains antibodies that bind to HCG which is connected to an indicator molecule that is usually a pigment the pigment antibody complex when the HCG enters the region it selectively binds with the antibody thus activating the indicator molecule the pigment is revealed in a line across the region indicating a positive result