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our project is to develop a new new method that we develop in the lab and apply it for epigenomic profiling to the goals of the 4d nucleon project yeah when you think about economic profiling of course youre going to think of chromatin immunoprecipitation so you might wonder well why why do we need a new method so let me just go through this even though chromatin immunoprecipitation is very familiar the way its generally practiced it really hasnt changed for some thirty years except for the last step and thats the readout so you start out by cross-linking cells and then sharing them usually by sonication and the antibody from unit precipitation and then you purify the DNA and chip cheek is been out there for now ten years and theres some 55,000 articles on it so its one of the most widely used methods and all of molecular biology but theres some limitations so let me go through these so first of all you need a lot of cells the efficiency is quite low the background is high and t