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if you are a molecular biology student dealing with a large number of DNA RNA or protein sequences then you must be familiar with the FASTA format, the FASTA format begins with a greater than symbol, followed by its ID and other details in the first line, the subsequent line denotes the actual sequence. when we download FASTA formatted files, then usually the sequences are shown in multiple lines rather than a single line. Thus it becomes very difficult to use the sequences for manipulation or analysis in Excel or worksheet using vlookup, sorting or other such functions. In this tutorial, with an example I will show you how to format a FASTA file, such that you get sequence in a single line. At the end of video, I will also share the Perl script. First you should have Perl program installed in your PC Next copy your file and script to the bin folder of the Perl here you see input.fa, which is my input file. It has FASTA sequences in multiple lines. This is the file which contai