Correct stain in PAP

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Upload your file to our editor. Choose how you prefer - dragging and dropping it into our uploading area, browsing from your device, the cloud, or using a secure URL to a third-party resource.
Start updating your PAP file. Use our toolbar above to add and edit text, or insert images, lines, icons, and comments.
Make more alterations to your work. Transform your PAP document into a fillable form with areas for text, dropdowns, initials, dates, and signatures.
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Share and save your form. Send your updated PAP file to other people as an email attachment, via fax, or create a shareable link for it - download or export your paperwork to the cloud with edits or in its initial version.

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How to Correct stain in PAP

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the fixation done between the 15 to 20 minutes of session we want to go to the next step which is the staining process okay before that we have to transfer from the fixed 95 alcohol slide into this slide holder [Music] right [Music] so please make sure that your slide will not dry up completely so you have to make it as soon as fast as possible okay so next we transfer this to the first [Music] weaker which is we transfer to another all right after 40 seconds we go to the third step which is the running tap water [Music] so please make sure the tap water is one running [Music] that means that for leave it for 40 seconds all right after 45 seconds done let me do the hot that which is hematology so we leave it for around 45 seconds okay after 45 second done we go to the next step which is the running tap for 40 seconds alright it for 40 seconds okay after 40 seconds the next step is we dip it in acid alcohol so this is one of the critical parts in the cleaning which if you over dip it it

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What is the best stain for cervical smear?

Pap stain is a universal stain used for gynecologic and non-gynecologic cytology smear. It is mainly used for oral and cervical cancer screening in asymptomatic population and in the follow up of patients with cancer. Pap test has decreased incidence of cervical cancer by 70% in developed countries.

What stains are used in PAP stain?

The PAP stain is a polychromatic counterstaining method consisting of stains such as Orange G 6 (OG6) and modified eosin azure (EA). The strength of the Pap stain is such that it results in: Well-stained nuclear chromatin.

What are the three main stains used in Pap stain?

The principle of pap stain Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid). Orange G is an acid dye and stains keratin with a bright intense orange.

Is Pap stain a Romanowsky stain?

Because the Papanicolaou stain was developed to document keratinization (the orange G dye component binds avidly to keratin), it is supe- rior to Romanowsky-type stains for this purpose. Identification of cytoplasmic keratinization may be essential for diagnosing squamous cell carcinomas.

What is the first stain used in Pap smear staining?

The first staining solution contains haematoxylin which stains cell nuclei. Papanicolaou used Harriss hematoxylin in all three formulations of the stain he published. The second staining solution (designated OG-6), contains Orange G in 95% ethyl alcohol with a small amount of phosphotungstic acid.

What is the EA 50 stain in PAPs for staining?

EA50 is a counter stain designed to stain the cytoplasm of cells derived from the human body. The stained cell sample is used to facilitate the diagnosis of the pathological state, and is intended for in-vitro examination of cellular deformity. PRINCIPLE OF METHOD: Papanicolaou staining (Pap.

How do you make Pap stain?

Basic Procedure for Papanicolaou Staining (Pap stain) Fix the smear with 95% Ethanol 15 minutes. Rinse in tap water. Add the Harris Hematoxylin dye for 1-3 minutes. Rinse in tap water or Scotts tap water. Dip the preparation in 95% Ethanol 10 dips. Add orange G-6 stain for 1.5 minutes. Dip in 95% Ethanol 10 dips.

What are the solutions of PAP stain?

Procedure of PAP stain Fix the smear immediately in 95% alcohol for 5-15 min. Take the smear to water (Hydration): 6 dips in each for the following solution of ethanol 80% (v/v) , 70%(v/v)and 50%(v/v) respectively. Rinse gently in distilled water. Stain in diluted Harriss hematoxylin for 2 to 3 minutes.

What materials are in pap stains?

The conventional Pap stain has 24 solutions, of which three are acid dyes, six water, one is chemically defined bluing agent, eight 95% ethanol, three absolute alcohol and three xylene Gill, 2006.

What are the 5 dyes in Pap stain?

The classic form of Pap stain involves five dyes in three solutions: A nuclear stain, haematoxylin, is used to stain cell nuclei. First OG-6 counterstain. The Orange G is used to stain keratin. Second EA (Eosin Azure) counterstain, comprising of three dyes; the number denotes the proportion of the dyes, eg.

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