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todays ask TaqMan question is how can you detect copy number variation with digital PCR sounds like an ideal application for the quants to do 3d digital PCR system but before I show you how first let me give you a little bit of background copy number variations commonly refer to a CNVs can result in having either too many or too few of a particular low sign like other types of genetic variation some cnvs may be responsible for a substantial amount of human phenotypic variability complex behavioral traits and susceptibility or resistance to disease when performing copy number variation with digital PCR youre performing an absolute quantification of the actual locus of interest to convert the absolute quantities to a copy number you want to compare your target to a reference locus that has not been amplified or changed in any way typically the reference will be diploid but it doesnt have to be it can be anything as long as the number of copies is known beforehand for CV determination
