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[Music] welcome to the applied biosystems tech talk video series where we break down real-time pcr to make it easier for you genotyping single nucleotide polymorphisms or snips using applied biosystems tacman assays is very well established but have you heard about copy number variation lets talk about it copy number variation or cnv is a type of structural variation that occurs when the number of copies of a particular dna segment deviates from the expected number of copies found in a reference genome these cnvs can influence gene expression and can be associated with specific phenotypes and diseases copy number variation covers approximately 12 percent of the human genome and includes deletions and duplications applied biosystems tacman copy number assays offer a targeted approach that combines high accuracy specificity ease of use and sample throughput to validate copy number changes or screen large sample sets so how does all this work takman copy number assays are duplex experime