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[Music] okay friends uh in this video Im going to discuss about uh the way or the typical mechanism of controlling the copy number of a plasmid now there are several different ways but this is one of the way of controlling the copy number in plasmid cells because the plasmid copy number regulation is really really important because the cell need to regulate the plasmid number at different times of their life cycle different times of their importance that for example a bacterial cell when it start to invade into uh some some host cell uh for the defense and all the mechanism they need to produce lot lot more amount of this plasmid and sometimes when they present in the dormant situations they need to uh lower down the plasmid production procedures thats why they need to control the amount of plasmid which are being produced and the number of plasmid uh to be really uh in in in in regulation okay now in this case we are talking about a mechanism of RNA interference uh for controlling o