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Today Ill be presenting AACR poster 6635 Enabling single cell analysis of copy number variation in breast cancer. For this case study we asked the question Can we call CNVs in clinical breast tissue samples? To do this, we purchased fresh frozen breast cancer tissue from Cureline which was Infiltrating Ductal Carcinoma grade two and we took three patient samples and processed it using our optimized internal nuclei prep protocol and well be highlighting one sample in particular for this presentation. Additionally we use an 8% spike-in of Raji to facilitate CNV baseline calling and for the panel we use our Tapestri Single Cell DNA Breast Cancer Research Panel which contains clinically relevant SNV and CNV and was validated by Jorge Reis-Filho at Memorial Sloan Kettering who is our inaugural Mission Bio Solid Tumor Center of Excellence. Here we have our SNV Calling and a schematic of our Tapestri pipeline output if youd like more information on this ple