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okay friends in this video i am going to discuss about the way or the typical mechanism of controlling the copy number of a plasmid now there are several different ways but this is one of the way of controlling the copy number in plasmid cells because the plasmid copy number regulation is really really important because the cell need to regulate the plasmid number at different times of their life cycle different times of their importance the for example a bacterial cell when it start to invade into uh some some host cell for the defense and all the mechanism they need to produce law lot more amount of this plasmid and sometimes when they present in the dormant situations they need to lower down the plasmid production procedures thats why they need to control the amount of plasmid which are being produced and the number of plasmid to be cut really uh in in in regulation okay now in this case we are talking about a mechanism of rna interference ah for controlling or of controlling this