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hi ashley here and today im going to show you how to make liquid culture liquid culture is a great way to speed up colonization and preserve genetics one downside to lcs is you cant see contamination so checking viability on agar is best to start we need to make the lids these lids are made of pp5 plastic which means that you can sterilize them in a pressure cooker they are 90 millimeters in size and fit on a 32 ounce mason jar these plastic lids are great because they dont rust like the metal ones do now we need to drill two holes one for the red injection port and one for the gas exchange filter for the gas to exchange port im using a 5 and 30 seconds drill bit and we just drilled them directly on the mason jar and it worked really well then insert the 13 millimeter filter into the hole and it should be a nice snug fit then for the self-healing injection port i used a half inch forstner drill bit then screw the lid on a mason jar and drill the la