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[Music] urine pregnancy test by card method the urine pregnancy test by card method is a qualitative immunoassay for detecting human chorionic gonadotropin or HCG hormone which is secreted in urine during pregnancy it enables detection of HCG for pregnancy as early as on the first day of a womans missed period our asper manufacturers recommended sensitivity these tests are easy to use versatile and produce rapid results principle when a woman becomes pregnant her body produces a hormone called HCG are the human chorionic gonadotropin which appears in the urine the secretion of this hormone increases throughout the first trimester HCG test utilizes the principle of immuno chromatography on a membrane as a test sample flows through the membrane assembly within the test device the colored conjugate binds with the HCG in the sample this complex moves further on the membrane to the test region where it is immobilized by the anti HCG antibodies coated on the membrane leading to the formatio