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the overall goal of the following experiment is to evaluate hippocampal dependent learning and memory in mice through the use of trace sphere conditioning this is achieved by first training the animal to associate an auditory tone with an aversive stimulus the next day the animal is placed in a novel context and freezing behavior is recorded in reaction to the tone on the third day the animal is returned to the original context and freezing behavior is recorded results show that mice trained with the shock tone pairing display more freezing behavior than those not receiving the shock this method can be used to provide insights into hippocampal dependent learning in memory in mice and is especially useful when used in the study of transgenic and knockout animals the following procedure is performed in a fear conditioning chamber housed inside a larger sound attenuating chamber with the door to the chamber closed position the lights to docHub the desired level of illumination if c
